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Waters hplc troubleshooting guide

 
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MessagePosté le: Sam 30 Déc - 19:12 (2017)    Sujet du message: Waters hplc troubleshooting guide Répondre en citant

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be flushed from the system daily with fresh deionized water. To isolate and repair specific problems related to your apparatus, use the troubleshooting and maintenance sections of the operation manual. Pump seals require periodic replacement. You should perform regular maintenance rather than waiting for a problem to.
HPLC troubleshooting - Hints and tips - More about chromatography. Secondary retention effects, normal-phase mode, Add triethylamine (basic compounds) or add acetic acid (acidic compounds) or add water (poly-functional compounds). Only for normal-phase methods Refere to detector manual to replace window.
This HPLC Troubleshooting Guide, written by Dr. Uwe Neue from the Waters Corporation, was developed to identify, explain, and solve some of the most common sources of laboratory problems in the areas of HPLC and Column Technology. There are over 50 topics for discussion in this guide which includ.
Reversed-phase chromatography - contaminated water, Check suitability of water by running different amounts through column and measure peak height of interferences as function of enrichment time; clean water by running it through old reversed-phase column; use HPLC-grade water. Unknown interferences in sample
29 Jul 2013 maintain, or troubleshoot the Waters Alliance e2695 Separations Module. This guide sets forth procedures for unpacking, installing, using, overview and maintenance guide, not operator's guide, accompanies the instrument, consult the instrument's online Help system for . HPLC system configurations .
Page 26. Column Connectors Used in HPLC. 0.090 in. 0.130 in. 0.090 in. 0.170 in. Swagelok. Waters. Parker. Rheodyne. Valco. Uptight. 0.090 in. 0.080 in. Troubleshooting LC Fittings, Part II. J. W. Dolan and P. Upchurch. LC/GC Magazine 6:788 (1988)
System Pressure Problems. – Incorrect Retention time . It should be define at the equipment user guide. – Compound and . Organic mixture. – Isopropanol-water + 1% acetic acid. ? Acid cleanup. – 30% phosphoric acid (~4.4N) for UPLC. Nitric acid for HPLC. – Use a last resort. Removal of all traces of acid with water is.
Successful HPLC. Operation. Analyze • Detect • Measure • Control™. C ? R ? m ? ? ? G R ? ? ? ?. A Troubleshooting Guide – Version 1.1 . system pressure under normal operating conditions each day or each time your HPLC is used. This will allow you to . 0.23 mm ID/1.58 mm OD tubing to the detector water outlet.
11. TROUBLE SHOOTING THE 2690/5. 2690/5 System Error Messages. MESSAGE. ACTION STEPS. Solvent delivery h/w fault. Cycle power. If message persists call Waters Service. Representative. Solvent delivery motor lost sync. Cycle power. Change inline filter. If message persists call. Waters Service Representative.
HPLC Troubleshooting. Uwe D. Neue compounds. Click on sections below to go directly to that section of the Troubleshooting Guide . used to get around this problem is to use non-polar solvents like hexane or methylene chloride that are half saturated with water. To accomplish this you take a volume of the solvent.

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MessagePosté le: Sam 30 Déc - 19:12 (2017)    Sujet du message: Publicité

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